Analysis of Slime Bacteria, Iron Bacteria, Silt Infiltration and Water Chemistry.

These tests help identify problems in wells due to slime forming bacteria, Iron bacteria, mineral deposits, corrosion, and odors in wells: and help determine if a problem is treatable long term.

For slime/iton bacteria problems: We recommend to do 2 testson a "Timed" basis to determine if the problem is contained (100% treatable) or coming from an outside source. Let the well set with no pumping for 8 hours. Take a "Casing" Sample within 30 seconds of vacating water in the pump. Take a second, "Aquifer" Sample after pumping 30 volumes of the well continuously. If a single test is done, we recommend a "Casing" Sample is done to understand the borehole environment. We can provide technical instructions or do the calculations for you.

For mineral deposits & corrosion problems: An "Aquifer" Sample taken at a minimum of continuously pumping 30 volumes of well will give you the actual aquifer conditions. If you want to understand borehole verses aquifer conditions, we recommend the "Timed" tests.

DWT Bacterial Analysis (500 ml or half quart bottles required)
2 "Timed" Bacterial Analysis (two tests on a timed basis)
This is a comparison of quantities of iron bacteria, slime formers and IDs to understand if the bacterial issues are contained in the borehole of the well (100% treatable, long term one time treatment with our chemistry) or coming from an outside source via failed grout, corrosion of the well casing, failed casing seat to a consolidated aquifer, etc. (untreatable long term). This includesa comparison of Heterotropic Plate Count, ID of bacteria with characteristics, ATP Count, anaerobic percentage, Sulfate Reducing Bacteria, ID/severity of iron oxidizing bacteria (iron bacteria), and inorganics pertinent to bacterial activity. See example.

DWT Single Bacterial Analysis (recommend a "Casing" Sample)
on either test, the full bacterial analysis is done.

Water Study® which includes bacterial assessment (1 liter or 1 quart bottles required)

2 "Timed" Water Study includes Water chemistry and DWT Bacterial Analysis

Single Water Study includes one Water Chemistry and DWT Baterial analysis
If a single sample, we recommend an "Aquifer" Sample done for accurate chemistry.

Single water chemistry only
Analysis of water chemistry can lead to understanding mineral deposits, treatment issues, and a potential for corrosion. This includes pH, manganese, iron, bicarbonate and carbonate hardnes, total hardness, silica, chlorides, total dissolved solids, sufates and phosphate. Calculation for Rysnar Stabilty index included.

Sampling and Shipping Instructions

Bacterial analysis requires 500 ml sample bottle. Our Water Study requires a 1000 ml bottle. Sample bottles are available at no charge, pre-labeled to our lab. Refrigerate samples if held overnight and then send via 1-2-3 day service. Do not ship samples for arrival to our lab on a Saturday. Ice packs are NOT necessary. If using your own containers, use clean, untreated, and washed bottles with the above sizes. See calculated times for sampling or call our Technical Service for assistance.

Send Water Samples - for analysis of water chemistry or bacteria to:

Jet-Lube, Inc. - Design Water Laboratory
4849 Homestead Rd.,
Suite 232
Houston, TX 77028


Call our Sales Office for pricing prior to sending.
Toll free in the US and Canada, 1-800-538-5823 or (713) 670-5700
for discussion of the problem, credit arrangements and billing.
No samples will be processed without prior approvals.

Explanation of Lab Services

Well yields may decline due to mineral deposits (water chemistry), slime/iron bacteria, silt/sand infiltration, or physical changes to the aquifer. Field diagnosis for mineral deposits or slime bacteria are listed in our booklet, "Understanding Well Problems & Pipelines". This may provide a direction for treatment at no cost. Our analysis is designed to identify problems before treatment.

For bacterial tests - iron bacteria, slime bacteria, odor problems, corrosion, and rising iron concentrations

DWT Bacterial Analysis

This includes 9 bacterial tests to include: 2 Biolog ID, counts per milliliter of aerobic growths (slime), anaerobic bacteria (odors/corrosion), Sulfate Reducing Bacteria (rotten egg odors or corrosion). ATP Count, and microscopic analysis (ID of iron bacteria/severity and physical debris. coliform count by SimPlate™ and the presence of absence of fecal coliform bacteria.

Explanation of Individual Tests

Biolog ID: The ID provides you with tendencies of bacteria plus the type and density of deposits.

Plate Count: Normal aquifer plate counts average in the tens of colonies per milliliter whereas when plugging problems exist in wells, numbers will often exceed hundreds to thousands of colonies/ml.

Anaerobic Growth: A high percentage of anaerobic bacteria indicates; 1- severe slime production with large deposits present. 2 - reasons for corrosion/odors. 3 - a reason for persistent coliform.

Sulphate Reducing Bacteria: (SRBs) test will provide an identification of SRBs. SRBs secrete a very corrosive enzyme and corrosion may be evident on drop pipes of pumps, pumps, casing, and screen. H2S gas is mainly produced by Sulfate Reducing Bacteria (SRBs) in groundwater which may even cause whitish water. SRBs can be naturally occurring in soil bacteria and often found in low Permeable areas (clay of shale) of an aquifer or in dead areas of a well (sumps). H2S odors in new wells can be treated with aeration. A sudden appearance of odors in older wells may be due to: 1) a new bladder style pressure tank replacing an older style galvanized air tank. The bladder entraps gas which doesn't escape until released at the faucet. 2) mineral scale or slime present in wells SRBs are anaerobic and hide in this debris. As chlorine oxidizes the outer layer of of debris, bacteria are exposed and odors can be extreme. SRBs can also cause corrosion, observed on metal parts.

ATP Test: (Adenosine Triphosphate) counts all live bacteria/ml. Normal aquifer counts will be in the low tens to hundreds of thousands. Numbers in the millions/ml may indicate massive growths and potential plugging. This test gives a second snapshot to the Biolog/Plate Analysis to determine severity of growth.

Microscopic Analysis: Identifies iron bacteria with severity, any other bacterial debris like decaying oxides, and physical debris present (sand, silts, etc.).

Coliform Count: Is done by SimPlate in colonies/100 ml. E. coli/Fecal coliform is identified as Positive-Negative. See example.

For water chemistry information and bacterial testing

Our "Water Study® determines the potential for plugging due to: 1) incrustation (mineral deposits) which includes corrosive vs. incrustive tendencies, 2) bacterial activity which includes DWT Bacterial Analysis (above). There is an option for just water chemistry. See example.

If mineral and bacteria are normal, reduction of yield may be due to silt infiltration or physical problems. If corrosion is evident and chemistry does not indicate corrosive tendencies, a bacterial analysis may help determine the potential for corrosion. If excessive corrosion is evident, stray DC voltage should be checked on site. With this, you have the option of doing water chemistry only.

Sludge or Debris Deposit Analysis: See "Sludge/Debris" analysis for directions for this service.


Well Rehab by Design, not by accident.